Volume 6 (2001) pp 649-675 |
Abstract |
An antimutagenic activity of fluphenazine, todralazine, anthocyanins
and alkylresorcinols was established in a battery of short-term cytogenetic tests.
One of the possible mechanisms of their antimutagenic action could be an
increase in apoptotic elimination of heavily-damaged cells from a culture. In
this paper we provide data on quantitative estimation of the antimutagensµ
impact on apoptosis in lymphocyte cultures exposed in the G0-phase to
genotoxic agents: hydrogen peroxide (0.2mM, 20 min.) or benzo[a]pyrene (40
����90 min.), and then cultured for 36 hrs in the presence of a lectin (PHA-M,
1% v/v) and each of the tested antimutagens. Apoptosis was estimated by means
of microscopic examination of cell smears stained with a mixture of
fluorochromes (ethidium bromide/acridine orange) as well as of the results of
DNA separation with the field inversion gel electrophoresis. By microscopic
examination we assessed that the frequencies of cells exhibiting morphological
features of apoptosis considerably increased in the cultures containing the
antimutagens. The FIGE separation of DNA from those cultures proved that the
DNA content in the 30-50 kb domain was markedly elevated, as compared with
the control cultures that did not contain antimutagens. It was established in the
regression analysis that the apoptosis-enhancing effect significantly depended
on the concentration of each tested antimutagen in a culture medium. However,
marked differences of apoptosis-enhancig potency were noticed among the four
antimutagens. The multicriterial analysis proved that the apoptosis-enhancing effects of fluphenazine and also, to a smaller extent, by alkylresorcinols, were
many times stronger than those of anthocyanins and of todralazine. The results
suggest that the enhancement of apoptosis by fluphenazine and by
alkylresorcinols can explain a major part of their antimutagenic activity,
whereas in the case of anthocyanins and of todralazine other mechanisms of
antimutagenic action should be sought for. |
Address and Contact Information |
1Wrocław Medical University, Department of Basic Medical Sciences,
Kochanowskiego 14, 51- 601 Wrocław, Poland, 2University of Wrocław,
Department of Genetic Biochemistry, Przybyszewskiego 63/77, 51-148
Wrocław, Poland, 3University of Wrocław, Department of Zoology,
Sienkiewicza 21, 50-335 Wrocław, Poland *Corresponding author, fax: (+4871) 3479211,e-mail: kaz@basmed.am.wroc.pl |