Vol. 4 No. 2 June 1999

Volume 4 (1999) pp 175-180
Title THE EFFECT OF WORTMANNIN AN PROMOTING PHORBOL ESTER (TPS) ON MIGRATION OF OVARY CARCINOMA CELLS
Authors Joanna Miłoszewska, Bożena Szaniawska, Dariusz Kowalczyk and Przemysław Janik
Abstract The purpose of the present study was to determine the role of PI 3 kinase in tumor cell migration. The migration of ovary carcinoma cells (OVP10) was strongly inhibited by wortmannin to the same extent as by TPA treatment. The strongest additive inhibitory effect was noted after cell treatment with wortmannin and TPA together. Western blotting studies showed activation / partial down regulation of PKC after TPA treatment and decreased amount of the enzyme following wortmannin exposure. Altogether, it looks like wortmannin inhibits migration of studied cells.
Address and Contact Information Department of Cell Biology, Cancer Center- Maria Skłodowska-Curie Institute of Oncology, 5,W.K. Roentgen str. 02-781 Warsaw, Poland.
[Rozmiar: 1332 bajtów]

Volume 4 (1999) 181-187
Title FORMATION OF DNA CROSSLINKS IN HUMAN LYMPHOCYTES BY ACETALDEHYDE REVEALED BY THE COMET ASSAY
Authors Janusz Błasiak*, Ewa Gloc-Fudała and Andrzej Trzeciak
Abstract Ethyl alcohol can be mutagenic, cancerogenic and teratogenic in man and its mutagenicity can be attributed to its first and major metabolite, acetaldehyde, which was reported to form adducts with DNA and proteins and DNA strand breaks. It was suggested that DNA crosslinks can be predominant DNA adducts of acetaldehyde but these results were obtained with the alkaline elution technique which provides no information on the extent of DNA damage at individual cell level. The comet assay is a technique, which allows detecting double- and single-strand DNA breaks caused by a broad spectrum of mutagens. It is also a suitable tool for the detection of crosslinking agents. Human peripheral blood lymphocytes were incubated for 1 h at 37°C with 3, 10, 30 and 100 mM of acetaldehyde. The alkaline comet assay was used to assess DNA damage. A dose-dependent decrease in the migration of DNA of acetaldehyde-treated cells was observed. Similar results were obtained when a recognized DNA crosslinking agent, formaldehyde was used. The results obtained suggest that acetaldehyde may form crosslinks with DNA in human lymphocytes. The nature of the crosslinks remains unknown and needs further investigations.
Address and Contact Information University of Łódź, Department of Molecular Genetics, Banacha 12/16, 90-237 Łódź, Poland
* corresponding author: Fax: +48 - 42 635 44 84, e-mail januszb@biol.uni.lodz.pl
[Rozmiar: 1332 bajtów]

Volume 4 (1999) pp 189-201
Title HORMONAL ACTIVITY AND MEMBRANE ACTION OF PLANTS TERPENOIDS
Authors Maria V. Zamaraeva1, Albert I. Hagelgans1, Larisa V. Lubnina, Andrey Y. Abramov1, Hafisa S. Ahmedhodjaeva2, Ashraf I. Saidhodjaev2, Natalia G. Glazyrina3and Bahtiar A. Salakhutdinov3
Abstract Terpenoids are a numerous class of natural compounds that possess various biological activities, including estrogenic action. In the present paper we report the results of study of estrogen and membrane activity of aromatic and aliphatic derivatives of sesquiterpen alcohol carotene, isolated from Ferula genus, directly testing the hypothesis that these compounds modulate hormone production via affecting the mobilisation of intracellular calcium. We have found that the complex ethers of sesquiterpen with aromatic but not with aliphatic substituents, more than 3-time increased the weight of uterus of prepubertal rats and stimulated the appearance of estrus in ovariectomized female rats in 80-100% of injected animals. Experiment carried out on intact cells (thymocytes) using the Ca2+indicator Fura-2/AM showed that terpenoids having stimulatory effect on the pubescence of rats increase [Ca2+]i. Furthemore, as observed in the experiments in vitro, these terpenoids dramatically inhibit Ca-ATPase and Na, K-ATPase of intracellular and plasma membranes and cause structural changes in membranes as was shown by EPR spectroscopy method. These data suggest that sesquiterpens could exert their estrogen action via increase intracellular Ca2+.
Address and Contact Information 1Department of Biophysics, Tashkent State University. 700174 Tashkent, Uzbekistan,
2Institute of Chemistry of Plant Substances, Academy of Science of the Republic of Uzbekistan, 700125 Tashkent, Uzbekistan,
3Institute of Bioorganic Chemistry, Academy of Sciences of the Republic of Uzbekistan, 700125,Tashkent, Uzbekistan
[Rozmiar: 1332 bajtów]

Volume 4 (1999) pp 203-218
Title INTERACTION OF SPECTRIN WITH PHOSPHOLIPIDS IS INHIBITED BY ISOLATED ERYTHROCYTE ANKYRIN. A MONOLAYER STUDY
Authors Katarzyna Białkowska, Jakub Leśniewski, Małgorzata Nietubyć and Aleksander F. Sikorski
Abstract It was previously found (Białkowska, K., Zembroń, A. and Sikorski, A.F. (1994) Biochim. Biophys. Acta 1191, 21-26.) that isolated erythrocyte ankyrin inhibited interaction of spectrin with phospholipid liposomes, mainly those prepared from lipid mixtures containing aminophospholipids. In this communication we report on the effect of isolated ankyrin on red blood cell spectrin interaction with phospholipids in monolayers. Our data indicate that spectrin interaction with monolayers containing PE and, to a smaller extent, PS is sensitive to inhibition by ankyrin while interaction with monolayers containing only PC is not. Tetrameric spectrin affects monolayer surface pressure similarly to the heterodimer. However, an interaction of tetrameric spectrin with phospholipids was much more effectively inhibited by purified ankyrin indicating that one site per spectrin tetramer engaged in this interaction. When interactions of purified individual spectrin subunits (a or b) with phospholipid monolayers was studied, the b-subunit caused a strong, saturable effect on the surface pressure of the PE/PC monolayer, in contrast to the a-chain which induced much smaller and monotonic changes on the surface pressure of the same monolayer. Also interactions of the b-subunit with amino-phospholipids/PC monolayers were more sensitive to inhibition by ankyrin than those with native ab-heterodimer of spectrin, e.g. threefold lower concentration of ankyrin was necessary to induce the same effect, while interaction of the a-subunit with phospholipid monolayers was entirely insensitive to ankyrin. Phosphorylation of spectrin in vitro with either cAMP-dependent protein kinase, or metabolically in intact cells, induced a decrease in the effect of either dimer or tetramer on the surface pressure of phospholipid monolayers. The sensitivity of this interaction to ankyrin was also greatly diminished. When isolated ankyrin was phosphorylated by the same cAMP-dependent protein kinase its ability to compete with phospholipid for spectrin was also diminished. The described effects may indicate a physiological significance of spectrin’s interaction with phospholipids, particularly in situations when there is not enough functional ankyrin to accommodate all spectrin molecules in the membrane.
Address and Contact Information University of Wrocław, Institute of Biochemistry, ul. Przybyszewskiego 63/77, 51-148 Wrocław, Poland
[Rozmiar: 1332 bajtów]

Volume 4 (1999) pp 219-302
Seminar Title W. MEJBAUM-KATZENELLENBOGEN'S MOLECULAR BIOLOGY SEMINARS 6. LIPOSOMES AND RELATED STRUCTURES
Date June 10-12, 1999, Wroclaw/Szklarska Poreba, Poland
Abstracts of the lectures
Abstracts List (TRANS-)ENDOTHELIAL TARGETING OF LIPOSOMES IN THE LIVER
G. L. Scherphof

THE HYDRATION OF LIPIDS AND WHAT IT CAN TELL US
G. Jendrasiak

MONOLAYERS AS MODEL MEMBRANES
P. Kinnunen

STRUCTURAL STUDIES ON THE POLYMORPHISM OF LIPIDS AND LIPID MIXTURES
G. Rapp

STUDIES ON THE MECHANISMS OF VESICLE FORMATION
B. Kloesgen

TOPOLOGY OF THE MEMBRANE INTERIOR
Y. Nakatani

BIOPHYSICAL ASPECTS OF LIPOSOMAL DELIVERY SYSTEMS
A. Fahr

BIOPHYSICAL PROPERTIES THAT CONTROL SIZE AND SHAPE OF EXTRUDED LIPOSOMES
K. Gawrisch

WHAT CAN LIPOSOMES TEACH US ABOUT HOW THE IMMUNE SYSTEM SEES ANTIGEN?
L. Leserman

PREPARATION AND CHARACTERIZATION OF CYTOTOXIC IMMUNOLIPOSOMES FOR THE SIMULTANEOUS INHIBITION OF ANGIOGENESIS AND TUMOR GROWTH
R. Schwendener

DIAGNOSTIC UTILITY AND PHARMACOKINETICS OF PEG-LIPOSOMES
G. Storm

SIGNAL TRANSDUCTION AS A TARGET FOR NEW CANCER THERAPIES
G. Lopez-Berestein

IN VIVO ANALYSIS OF METASTATIC PROCESS AND SUPPRESSION OF METASTASIS
N. Oku

CATIONIC PHOSPHOLIPIDS: PHYSICAL PROPERTIES, COMPLEXES WITH DNA AND TRANSFECTION ACTIVITY.
R. MacDonald

BIOCHEMICAL AND FUNCTIONAL ANALYSES OF A PEPTIDE/PHOSPHOLIPID/DNA TERNARY COMPLEX USED FOR GENE DELIVERY
J. -Y. Legendre

SYNTHETIC AMPHIPHILES AS NON-VIRAL VECTORS FOR GENE-THERAPY: DEVELOPMENT AND MECHANISMS
D. Hoekstra

ANNAMYCIN, A NOVEL LIPOSOMALLY FORMULATED ANTICANCER AGENT
W. Priebe

ELECTRICALLY ASSISTED TRANSDERMAL DRUG DELIVERY OF LIPOSOMAL FORMULATIONS
G. Betageri

INCREASE OF THE ANTITUMOR EFFECT OF LIPOSOMAL OCTADECYL-PIPERIDINOYL-PHOSPHATE: INFLUENCE OF COMPOSITION ON THE FINAL EFFECT
R. Zeisig

SUB-MICRON LIPID EMULSIONS AS DRUG CARRIERS FOR ANTI-CANCER DRUGS
B. Lundberg

CHARACTERIZATION OF SOLID LIPID NANOPARTICLES BY EPR
M. Sentjurc

PLAROSOMES, NOVEL EFFICIENT PHOSPHOLIPID-AMPHIPHILE VESICLES FOR DRUG DELIVERY
Arkadiusz Kozubek, Jerzy Gubernator, Ewa Przeworska and Maria Stasiuk
  Full text of the abstracts
Oral presentations
Abstracts List EFFECTS OF ANGIOTENSIN DERIVATIVES (LANG) - FILLED LIPOSOMES ON ISOLATED RAT AORTA.
Marcel Costuleanu, Natalia Costuleanu, Liliana Foia, Simona - Mihaela Slatineanu, Angela Costuleanu, Gheorghe Petrescu

MODULATION OF FUNCTIONAL PROPERTIES OF ARTIFICIALLY HYDROPHOBIZED a(alpha)-CHYMOTRYPSIN BY INCORPORATION INTO LIPID BILAYER.
N.O.Korobova, I.B.Bruskovskaya, N.S. Melik-Nubarov, A.A.Yaroslavov

LIPOSOMES AS MODEL FOR THE INTERACTION OF TEMPORINS AND PHOSPHOLIPID BILAYERS.
Antonio Di Giulio, Andrea C. Rinaldi, Giancaterino Gualtieri, Manuela Liberi, Donatella Barra, Maurizio Simmaco and Argante Bozzi

CONFORMATIONAL TRANSITIONS IN PHOSPHOLIPID BILAYERS: EFFECT OF PACKING FORCES AND HYDRATION.
Karol S. Bruzik

CYTOPLASMIC DELIVERY BY SULFATIDE-CONTAINING LIPOSOMES TO CELLS IN CULTURE.
Qiu-Tian Li and Xiaofeng Wu

ON THE TRANSPORT OF SOME ECOTOXICANTS THROUGH LIPOSOMAL MEMBRANES.
E.A. Saratovskih, T.A.Kondratieva, B.L. Psikha

  Full text of the abstracts
Poster abstracts
Abstracts List FEASIBILITY OF IN VIVO MAGNETOLIPOSOME TARGETING.
M. Babincová, V. Altanerová, M. Lampert, C. Altaner, E. Machová, M. (c)rámka and P. Babinec

TOPICAL DRUG RELEASE FROM LIPOSOMES USING LASER PULSES.
M. Babincová, P. Sourivong, D. Leszczynska and P. Babinec

NEOGLYCOCONJUGATES-A TOOL FOR CONJUGATION OF CARBOHYDRATES WITH LIPOSOMES.
Janusz Boratyński

IMMUNOLIPOSOMAL-DNA COMPLEXES TARGETED TO T CELLS.
B.Carrington, C.Catterall, D.T.Brown, A.Turner, P.Antoniw, T.Baker, N.Weir.

ANNEXIN VI, A NOVEL ATP-DEPENDENT PROTEIN. ALTERA-TIONS OF PHOSPHOLIPID BINDING PROPERTIES OF ANNEXIN VI BY NUCLEOTIDES.
M. Danieluk, A.F. Sikorski, S. Pikuła, J. Bandorowicz-Pikuła

PREPARATION OF PEG-GRAFTED IMMUNOMAGNETO-LIPOSOMES AND THEIR APPLICATION IN CELL SORTING.
J.C. Domingo, M. Mercadal and M.A. de Madariaga

EFFECT OF STARBURST PAMAM DENDRIMERS ON ERYTHROCYTE MEMBRANE PROTEINS.
Miłosz A. Faber, Dariusz Domański, Maria Bryszewska, Wanda Leyko

THE PROTECTIVE EFFECT OF QUERCETIN-5-SULPHONIC ACID ON THE MEMBRANE ORGANOTIN ADSORPTION.
Janina Gabrielska, Marek Langner, Stanisław Przestalski

PLAROSOMES - A NEW CONCEPT FOR ANTHRACYCLINE DRUGS DELIVERY.
Jerzy Gubernator, Ewa Przeworska, Arkadiusz Kozubek

INTERACTION OF CYSTEINE PROTEINASE INHIBITOR WITH LIPOSOMES. I. FLUORESCENCE STUDIES.
Jan Gutowicz, Andrzej Poła, Jousif Saleh, Maciej Siewiński

INTERACTION OF CYSTEINE PROTEINASE INHIBITOR WITH LIPOSOMES. II. EFFECT ON THE INHIBITION OF CATHEPSIN.
Jan Gutowicz, Jousif Saleh, Andrzej Poła, Maciej Siewiński

CYCLIC VOLTAMMETRY AND FLUORESCENCE SPECTRO-SCOPY STUDIES ON THE EFFECT OF POLYSIALIC ACID ON PHOSPHOLIPID MODEL MEMBRANES.
Teresa Janas, Krzysztof Nowotarski and Tadeusz Janas

TRANSLOCATION OF POLYSIALIC ACID ACROSS LIPOSOMAL MEMBRANES: ENERGY BARRIER MODEL AND ELECTRON MICROSCOPY STUDIES.
Tadeusz Janas, Henryk Krajiński and Teresa Janas

COMPARATIVE STUDIES ON THE EFFECT OF SIDE CHAIN LENGTH OF PLASTOQUINONES AND PLASTOQUINOLS ON THEIR INTERACTION WITH PHOSPHOLIPID BILAYERS.
Jemioła-Rzemińska M. , Myśliwa-Kurdziel B. and Strzałka K.

INFLUENCE OF BOLAAMPHIPHILIC STEROID DIMER ON FORMATION AND STRUCTURE OF BILAYER LIPID MEMBRANES.
Sławomir Kalinowski, Zenon Łotowski, Jacek W. Morzycki

CHOLESTEROL-INDUCED VARIATIONS IN PORE FLUCTUA-TIONS OF BILAYER LIPID MEMBRANE.
Stanisława Koronkiewicz, Krzysztof Bryl

REGULATION EFFECT OF AMPHIPHILIC ANIONIC COMPOUNDS ON CALCIUM ION DESORPTION PROCESS FROM LECITHIN LIPOSOME MEMBRANES MODIFIED BY TRIPRO-PYLTIN AND TRIPROPYLLEAD COMPOUNDS.
Teresa E. Kral, Janina Kuczera, Stanisław Przestalski

PREPARATION OF MEO-PEG-DPPE SURFACE LIPOSOMES MODIFYING AGENT.
Leszek Krzyżanowski

EXPERIMENTAL AND THEORETICAL STUDIES ON FLUORESCEINE-PE IN DPPC BILAYER.
K. Kubica, M. Langner

FORMATION OF LIPID-FURAGINUM COMPLEXES.
M. Langner, K. Kubica and M. Adamkiewicz

THE EFFECT OF LIPID BILAYER ORGANIZATION ON THE ADSORPTION OF ORGANOMETALLIC, AMPHIPHILIC MOLECULES.
Marek Langner, Janina Gabrielska, Stanisław Przestalski

VIOLAXANTHIN DE-EPOXIDATION IN MODEL LIPID MEMBRANES.
Dariusz Latowski, M. Skrzynecka, J. Kruk, A. Kostecka, K. Strzałka

IMMUNOLIPOSOMES FOR THE SIMULTANEOUS TARGETED INHIBITION OF ANGIOGENESIS AND TUMOR GROWTH.
Cornelia Marty, Kurt Ballmer-Hofer, Dario Neri, Roman Klemenz, Reto A. Schwendener

TIME AND TEMPERATURE DEPENDENCE OF LIPOSOME UV SPECTRA.
Anna Michnik, Zygmunt Zawada

SPHINGOMYELIN-CHOLESTEROL LIPOSOMES. EFFECT OF NATURAL RESORCINOLIC AMPHIPHILES ON THEIR PROPERTIES.
Ewa Przeworska, Jan A.A.M. Kamps, Gerrit L. Scherphof, Arkadiusz Kozubek

THE EFFECT OF SOME PHENYLTIN COMPOUNDS ON THERMOTROPIC BEHAVIOUR OF MIXED PHOSPATIDYL-CHOLINE/ CHOLESTEROL BILAYERS.
Bożenna Różycka - Roszak, Hanna Pruchnik, Eugeniusz Kamiński

COUNTERION EFFECT ON MICELLIZATION AND INTERACTION WITH PHOSPHOLIPID BILAYER.
Bożenna Różycka - Roszak, Adriana Mucha, Romuald Żyłka

SURFACE MODIFIED LIPOSOMES BY COATING WITH CHARGED HYDROPHILIC MOLECULES.
M. Luisa Sagristá, Margarita Mora and M. Africa de Madariaga

INFLUENCE OF REDOX AGENTS ON ACTIVITY OF PROTEIN TYROSINE PHOSPHATASES AND KINASES IN HUMAN AORTIC ENDOTHELIAL CELLS.
B.Zieliński, E.Seweryn, T. Banaś

NON-COVALENT INTERACTIONS OF LIPOSOMES AND IgE.
Zygmunt H. Zawada

ONE-STEP PROCESS FOR PREPARATION OF HOMOGENEOUS LIPOSOMES.
Zygmunt H. Zawada, Leszek J. Krzyżanowski

  Full text of the abstracts